Allergy Methods and Protocols [Paperback]

Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.

In recent years, allergy research has focused on the causes and mechanisms of allergy. In parallel, there is also an impetus to try to understand mechanisms of natural tolerance and immunotherapy where allergy is being dampened. In Allergy: Methods and Protocols a groundbreaking new title from the Methods in Molecular Medicine series, leaders in the field provide assistance for researchers to gain insight into the molecular mechanisms involved in allergy by featuring an array of protocols. These cover a range of disciplines including allergy, immunology, cell biology and histology and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, T regulatory cells. Techniques discussed include; B and T cell epitope mapping, characterization of allergens, conjugation of haptens, preparation of monoclonal antibodies, collection and sampling of airborne allergens, IgG antibodies and facilitated antigen blocking assays, identification and purification of mast cells and in situ hybridisation. Allergy: Methods and Protocols will be a remarkably useful bench tool for anyone embarking in or continuing with their research in allergy.

List of chapters1. Understanding the molecular mechanisms of allergy - Meinir Jones2. T cell - primary culture from peripheral blood - MonikaRaulf-Heimsoth3. Production of T cell lines - Helga Kahlert4. Production of human T cell clones - Adrienne Verhoef5. Mapping of human T-cell epitopes of allergens - Thomas Zeiler andTuomas Virtanen6.l£‡