Alpha-1 Antitrypsin Deficiency Methods and Protocols [Hardcover]

This volume provides protocols that expand on the latest alpha-1-antitrypsin (AAT) research. The chapters in this book are divided in to three sections: Part I is dedicated to patient-oriented research; part II discusses animal models; and Part III focuses on in vitro studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Alpha-1 Antitrypsin Deficiency: Methods and Protocols is a valuable resource for researchers, students, and clinican-scientists interested in AAT deficiency, as well as anyone working in the fields of pulmonology and hepatology.

1. Pathophysiology of Alpha-1 Antitrypsin Deficiency Liver Disease
JeffreyH. Teckman and Keith S. Blomenkamp

2. Pathophysiology of Alpha-1 Antitrypsin Lung Disease
Michael Kalfopoulos, Kaitlyn Wetmore, and Mai ElMallah

3. Measuring and Interpreting Serum AAT Concentration
Leslie Donato, Melissa Snyder, and Dina N. Greene

4. AAT Phenotype Identification by Isoelectric Focusing
Dina N. Greene, M.C. Elliot-Jelf, and David G. Grenache

5. Laboratory Diagnosis by Genotyping
Irene Belmonte, Luciana Montoto, and Francisco Rodr?guez-Fr?as

6. Genotyping Protocol for the Alpha-1 Antitrypsin (PiZ) Mouse Model
Alisha M. Gruntman&ll³g