Auditory and Vestibular Research: Methods and Protocols [Hardcover]

Thissecond edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methodsand Protocols, Second Edition guides readers through protocols on cellculture, tissue engineering, nanotechnology, high-throughput screening, andphysiology.  Chapters on physiology covertechniques that include optical coherence tomography, patch clamping, andphotostimulation of caged neurotransmitters. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritativeand cutting-edge, Auditoryand Vestibular Research: Methods and Protocols, Second Edition aims to ensure successfulresults in the further study of this vital field.

 Helios?Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: RecentUpdates.- Tol2-MediatedDelivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation.-  A Rapid,Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation EnablesEfficient Gene Transfer to the Developing Mouse Inner Ear.-  Generationof Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannomaand Meningioma.- RNAExtraction from Xenopus Auditory and Vestibular Organs for Molecular Cloningand Expression Profiling with RNA-Seq and Microarrays .- YeastTwo-Hybrid Screening to Test for Protein-Protein Interactions in the AuditorySystem.-  MultiplexedIsobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches toAuditory Research.- ProteinQuantification of the Developing Cochlea by MalE