Bone Marrow and Stem Cell Transplantation [Hardcover]

The second edition of Bone Marrow and Stem Cell Transplantation expands upon the previous edition with current, detailed methods on HLA, minor-HLA and Killer Immunoglobulin Like Receptor typing. With new chapters on immunophenotyping and functional characterization of stem cells are included. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Bone Marrow and Stem Cell Transplantation, Second Edition serves as a guide in the application of molecular methods for routine or investigational purposes.

1. Reporter Gene Technologies for Imaging Cell Fates in Hematopoiesis

Sophie Kusy and Christopher H. Contag

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2. Flow Cytometry for Hematopoietic Cells

Daniela S. Krause, Michelle E. DeLelys, Frederic I. Preffer

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3. Isolation and Characterization of Mesenchymal Stem Cells

Sedat Odabas, A. Eser Elcin, and Y. Murat Elcin

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4. Methods for Functional Analysis of Stem Cells

Aurore Saudemont, Michelle Escobedo-Cousin and J Alejandro Madrigal

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5. An Overview of HLA Typing for Heamatopoietic Stem Cell Transplantation

Katy Latham, Ann-Margaret Little, J Alejandro Madrigal

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6. HLA Typing with Sequence-Specific Oligonucleotide Primed PCR (PCR-SSO) and Use of the Luminex Technology

Klara Dalva and Meral Beksac

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7. Sequence Based Typing of HLA: An Improved Group SpecifilC¯