Branched-Chain Amino Acids, Part B [Hardcover]

Volume 324 of Methods in Enzymology supplements Volume 166. It includes genetic information (cloning, gene expression) and information on human genetic diseases not available when Volume 166 was published.
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The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

• Preparation of substrates and assay of enzymes
• Cloning, expression, and purification of enzymes
• Detection and consequences of genetic defects
• Regulation and expression of enzymes

Section I: Preparation of Substrates, Assays of Intermediates and Enzymes, and Use of Enzyme Inhibitors

[1]: Synthesis and Gas Chromatography/Mass Spectrometry Analysis of Stereoisomers of 2-Hydroxy-3-methylpentanoic Acid

[2]: Analysis of Intracellular Metabolites as Tool for Studying Branched-Chain Amino Acid Biosynthesis and Its Inhibition in Bacteria

[3]: Determination of Branched-Chain L-Amino-Acid Aminotransferase Activity

[4]: Analysis of (S)- and (R)-3-Methyl-2-oxopentanoate Enantiomorphs in Body Fluids

[5]: Spectrophotometric Assay for Measuring Branched-Chain Amino Acids

[6]: Determination of Branched-Chain ?-Keto Acid Dehydrogenase Activity State and Branched-Chain ?-Keto Acid Dehydrogenase KinaselÃs