Candida Species Methods and Protocols [Hardcover]

This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

PART I: Molecular Analyses Of Pathogenesis

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1 How to use the Candida Genome Database

Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock

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2 Transcriptional Profiling of Candida albicans in the Host

Kearney T.W. Gunsalus, Carol A. Kumamoto

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3 SRYTH: A New Yeast Two-Hybrid Method

Jaideep Mallick, Gregor Jansen, Cunle Wu and Malcolm Whiteway

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4 Chromatin immunoprecipitation (ChIP) assay in Candida albicans

Sreyoshi Mitra,? Laxmi Shanker Rai,? Gautam Chatterjee, ?and Kaustuv Sanyal

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5 Assessing mitochondrial functions in Candida albicans

Richard Calderone and Dongmei Li

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6 ?Imaging Candida Infections in the Host

Dhammika H. Navarathn, David D. Roberts, Jeeva Munasinghe, ?and Martin J. Lizak

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7 Identification of Secreted Candida Proteins using Mass Spectrometry

Emilia G?mez-Molero, Henk L. Dekker, Albert lS"