Cervical Cancer Methods and Protocols [Hardcover]

Representing the most relevant procedures and technologies aiding the advance of the field of HPV-mediated carcinogenesis of the cervix and other anatomical regions of squamocolumnar transition, such as the anorectum, penis, and oropharynx, Cervical Cancer: Methods and Protocols compiles a detailed collection of practical chapters. The first half of the book covers HPV types, pathogenesis of cervical cancer (CxCA), prevention, and novel potential drug targets, while the second half explores pathology, genomics, modeling of CxCA, and experimental therapeutic strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and vital, Cervical Cancer: Methods and Protocols serves as a valuable resource to both bench scientists and clinicians who step into the realm of high-risk HPVs and CxCA for the first time or those who wish to learn novel approaches or expand their toolbox for the study of CxCA.

Part I: Approaches to the Detection and Analysis of HPV Types

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1. Evolution and Classification of Oncogenic Human Papillomavirus Types and Variants Associated with Cervical Cancer

??????????? Zigui Chen, Luciana Bueno de Freitas, and Robert D. Burk

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2. A Real-Time PCR Approach Based on SPF10 Primers and the INNO-LiPA HPV Genotyping Extra Assay for the Detection and Typing of Human Papillomavirus

??????????? M. Isabel Micalessi, Gaelle A. Boulet, and J. Jean-Paul Bogers

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Part II: Molecular Pathogenesis of CxCA

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3. Replication of Huml#&