CRISPR: Methods and Protocols [Hardcover]

This volume presents a list of cutting-edge protocols for the study of CRISPR-Cas defense systems and their applications at the genomic, genetic, biochemical and structural levels.?CRISPR: Methods and Protocols?guides readers through techniques that have been developed specifically for the analysis of CRISPR-Cas and techniques adapted from?standard protocols of DNA, RNA and protein biology.?Written in the highly successful?Methods in Molecular Biology?series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge,?CRISPR: Methods and Protocols?provides a broad list of tools and techniques to study the interdisciplinary aspects of the prokaryotic CRISPR-Cas defense systems.

1. Investigating CRISPR RNA Biogenesis and Function using RNA-seq

Nadja Heidrich, Gaurav Dugar, J?rg Vogel, and Cynthia M. Sharma

?

2. In vitro Co-reconstitution of Cas Protein Complexes

Andr? Plagens and Lennart Randau

?

3. Analysis of CRISPR pre-crRNA Cleavage

Erin L. Garside and Andrew M. MacMillan

?

4. Annotation and Classification of CRISPR-Cas Systems

Kira S. Makarova and Eugene V. Koonin

?

5. Computational Detection of CRISPR/crRNA Targets

Ambarish Biswas, Peter C. Fineran, and Chris M. Brown

?

6. High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium