Cyclic Nucleotide Signaling in Plants: Methods and Protocols [Paperback]

Over the last two decades there has been a growing interest in cyclic nucleotide research in plants with an emphasis on the elucidation of the roles of cGMP and cAMP. In Cyclic Nucleotide Signaling in Plants: Methods and Protocols, expert researchers in the field detail many approaches to better understand the biological role of this important signaling system. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and practical, Cyclic Nucleotide Signaling in Plants: Methods and Protocols seeks to aid scientist in further understanding signal transduction and the molecular mechanisms underlying cellular signaling.

1: Molecular Methods for the Study of Signal Transduction in Plants

Helen R. Irving and Chris Gehring

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2: Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains

Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi

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3: Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

Heike Burhenne and Volkhard Kaever

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4: Determination of ADP-ribosyl CyclalãÀ