Enzymology at the Membrane Interface Intramembrane Proteases [Hardcover]

Enzymology at the Membrane Interface: Intramembrane Proteases, Volume 584, the latest release in the Methods in Enzymology series, covers a subset of enzymes that work in the environment of the biological cell membrane. This field, called interfacial enzymology, involves a special series of experimental approaches for the isolation and study of these enzymes.

• Covers a subset of enzymes that work in the environment of the biological cell membrane
• Offers a series of experimental approaches for the isolation and study of enzymes

1. Biochemical Characterization of Function and Structure of RseP, an Escherichia Coli S2P Protease Y. Hizukuri, K. Akiyama and Y. Akiyama 2. Signal Peptidase Enzymology and Substrate Specificity Profiling R.E. Dalbey, D. Pei and ?.D. Ekici 3. Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins S. Veugelen, M. Dewilde, B.D. Strooper and L. Ch?vez-Guti?rrez 4. Probing the Activity of Eukaryotic Rhomboid Proteases in Vitro B. Cordier and M.K. Lemberg 5. Expression, Purification, and Enzymatic Characterization of Intramembrane Proteases R. Zhou, Y. Shi and G. Yang 6. Analyzing Amyloid-? Peptide Modulation Profiles and Binding Sites of ?-Secretase Modulators J. Trambauer, A. Fukumori, B. Kretner and H. Steiner 7. Probing the Structure and Function Relationships of Presenilin by Substituted-Cysteine Accessibility Method T. Tomita 8. A New Method to Determine the Transmembrane Conformation of Substrates in Intramembrane Proteolysis by Deep-UV Resonance Raman Spectroscopy J.W. Cooley, A. Abdine, M. Brown, J. Chavez, B. Lada, R.D. JiJi and I. Ubarretxena-Belandia 9. An Inducible Reconstitution System for the l3%