Epithelial Cell Culture: Methods and Protocols [Hardcover]

This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and  two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test  cell activities. Further,  The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.

2.      Bovine mammary organoids: a model to study epithelial mammary cells 

3.      Equine primary granulosa cell culture: analyses of transcriptome

4.      Amniotic epithelial cells culture

5.      Models of the small and large Intestinal epithelium: epithelial cell coculture

6.      Differentiation ol£§