Expression Profiling in Neuroscience [Hardcover]

Transcriptomics and proteomics, studying the profile of the expression of nucleic acids and proteins respectively, are increasingly applied to gain a mechanistic insight into a wide spectrum of investigation, and the use of expression profiling studies for the central nervous system and brain function aids in the understanding of neurodegenerative disorders and tumor development mechanisms.? In Expression Profiling in Neuroscience, expert researchers provide a survey of the most commonly used approaches in the field and scan the different ways of studying the central nervous system/brain environment through expression profiling.? The first part addresses the gene expression profiling of the brain at a large scale or to a specific cell type such as blood-brain barrier endothelium. Then, the second part describes the protein expression studies and the different technologies applied.? Written for the popular Neuromethods series, chapters include corresponding background information, tested laboratory protocols, and step-by-step methods for reproducible laboratory experiments.

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Detailed and authoritative, Expression Profiling in Neuroscience presents the state-of-the-art techniques necessary to expand research further into this vital area of study.

Transcriptomics and proteomics are increasingly deployed in a variety of investigations, including research into brain disorders. Cutting-edge and highly practical, this book sets out easily reproducible methods of both gene- and protein expression profiling.

1. DNA Microarrays for Gene Expression Analysis in Brain Tissue and Cell Lines

??????????? Gustavo A. Barisone and Elva D?az

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2. Gene Expression Profiling Using the Terminal Continuation (TC) RNA Amplification Metholӟ