Flow and Image Cytometry [Paperback]

Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I, Membrane Dynamics and Function , deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton, etc. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The third part covers data management systems, cell sorting techniques and microscopy.Proceedings of the NATO Advanced Study Institute Progress in Flow and Image Cytometry , held at Villejuif, France, May 15-19, 1995Image analysis and flow cytometry are complementary techniques which provide new insights into essential aspects of cell biology. This book presents an up-to-date overview of current ideas and methods in these domains. It consists of three parts. Part I, Membrane Dynamics and Function , deals with adhesion molecules, membrane pump dynamics, membrane fusion and endocytosis, membrane potential, T-cell homing and cytoskeleton, etc. The second part focuses on problems of cell proliferation, chromosome analysis and sorting, apoptosis, imaging cytometry of fixed and living cells, image analysis for gene mapping from flow-sorted chromosomes, chromatin organization, and liposome-mediated delivery of DNA binding drugs. The third part covers data management systems, cell sorting techniques and microscopy.I: Membrane Dynamics and Function.- Analysis of CD28 Interactions with Its Cognate Counter-Receptors CD80 and CD86.- Membrane Transport Dynamics.- Endogenous Lectins in Circulating Cells and Their Glycosylated Ligands: Their Role in T Cell Homing.- Use oflC$