Fluorescence Spectroscopy and Microscopy: Methods and Protocols [Paperback]

Reflecting the expanding fields need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject.? The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS).? Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results.

?

Part I: Steady-State Fluorescence Spectroscopy (SSFS)

?

1. How to Collect National Institute of Standards and Technology (NIST) Traceable Fluorescence Excitation and Emission Spectra

??????????? Adam Matthew Gilmore

?

2. Steady State Fluorescence Polarization/Anisotropy for the Study of Protein Interactions

??????????? Nicholas G. James and David M. Jameson

?

3. Quantitative Fluorescence Spectral Analysis of Protein Denaturation

??????????? Ivo H.M. van Stokkum and Sergey P. Laptenok

?

4. High-Pressure Fluorescence Applications

??????????? Mariano Dellarole and Catherine A. Royer