Gene Essentiality: Methods and Protocols [Hardcover]

This volume opens by covering two main types of approaches widely used to determine essential genes: single-gene knockouts and transposon mutagenesis, in both prokaryotes and Candida albicans. Given the significant advancement in the computational predictions of microbial essential genes, the second half of the book examines four main types of approaches: comparative genomics, supervised machine learning, constraint-based methods, and corrections of transposon mutagenesis data, as well as databases and servers that are often used in studying gene essentiality. Written in the highly successful Methods in Molecular Biology series format, chapters include an introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Gene Essentiality: Methods and Protocols will aid researchers who wish to further our knowledge in this vital field of study.

1. Microarray Transposon Tracking for the Mapping of Conditionally Essential Genes in Campylobacter jejuni

??????????? Martin Stahl and Alain Stintzi

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2. Identifying Essential Streptococcus sanguinis Genes Using Genome-Wide Deletion Mutation

??????????? Lei Chen, Xiuchun Ge, and Ping Xu

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3. Defining Essential Genes and Identifying Virulence Factors of Porphyromonas gingivalis by Massively-Parallel Sequencing of Transposon Libraries (Tn-seq)

??????????? Brian A. Klein, Margaret J. Duncan, and Linden T. Hu

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4. Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12

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