Gene Expression Profiling: Methods and Protocols [Hardcover]

Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease.? In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

?

In this book, experts present a broad range of available and developing techniques for the analysis of gene expression at the DNA, RNA, and protein levels. A collection of clearly described and illustrated chapters includes tips and implementation advice.

1. Real-Time PCR and Multiplex Approaches

??????????? Olga L. Gurvich and Mikhail Skoblov

?

2. Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs

??????????? Sweta Rani and Lorraine ODriscoll

?

3. Microarray Analysis of mRNAs: Experimental Desiló´