Gene Expression Profiling [Paperback]

Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip?, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay?, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip?, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay?, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.Technical Considerations in Quantitating Gene ExpressionRichard A. ShimketsGene Expression Quantitation Technology SummaryRichard A. ShimketsStandardized RT-PCR and the Standardized Expression Measurement CenterJames C. Willey, Erin L. Crawford, Charles R. Knight, K. A. Warner, Cheryl A. Motten, Elizabeth A. Herness, Robert J. Zahorchak, and Timothy G. GravesGeneCalling: Transcript Profiling Coupled to a Gene Database QueryRichard A. ShimketsInvader Assay for RNA QuantitationMarilyn C. Olson, Tsetska Takova, LuAnne Chehak, Michelle L. Curtis, Sarah M. Olson, and Robert W. KwiatkowskiMonitoring Eukaryotic Gene Expression Using Oligonucleotide MicroarraysJennifer Lescallett, Marina E. Chicurel, Robert Lipshutz, and Dennise D. Dalma-WeiszhauszAmplified Differential Gene Expression Microarray, Zhijian J. ChenlÖ