Gene Expression Systems Using Nature for the Art of Expression [Hardcover]

Gene Expression Systems: Using Nature for the Art of Expression offers detailed information on a wide variety of gene expression systems from an array of organisms. It describes several different types of expression systems including transient, stable, viral, and transgenic systems. Each chapter is written by a leader in the field. The book includes timelines and examples for each expression system, and provides an overview of the future of recombinant protein expression.

• Provides detailed information on expression systems
• Covers a variety of promoters and host organisms enabling researchers to tailor protocols to their specific needs
• Includes timelines and examples
• Compares pros and cons of each method

Contributors.
Introduction: The Art of Expression.
Purpose of This Book.
Selecting a Suitable Expression System:
Considerations.
Genomics and the Future of Protein Expression Systems, J.M. Fernandez and J.P. Hoeffler.
Prokaryotic Expression Systems:
Gene Expression Systems Based on Bacteriophage T7 RNA Polymearse, R. Durbin.
Expression Vectors Employing the trc Promoter, J. Brosius.
Bacillus Expression: A Gram Positive Model, E. Ferrari and B. Miller.
araB Expression System in Escherichia coli, M. Better.
Eukaryotic Expression Systems:
Adenoviral Vectors for Protein Expression, D.J. von Seggern and G.R. Nemerow.
Expression in the Methalotrophic Yeast Pichia pastoris, J.M. Cregg.
Recombinant Protein Expression in Pichia methanolica, C.K. Raymond.
Cytomegalovirus PromlÓ$