Glioblastoma: Methods and Protocols [Hardcover]

This volume details cutting-edge protocols on the characterization of the genome, epigenome, proteome, metabolome and single-cell transcriptome of tumors and tumor-derived cultures. Chapters focus on subpopulations of cells with stem-like properties, laser capture microdissection, and modeling human glioma with human embryonic stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Glioblastoma: Methods and Protocols aims to support researchers seeking new and refined protocols to better decrypt this tumor.?

1. Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma

Kazimierz O. Wrzeszczynski, Vanessa Felice, Minita Shah, Sadia Rahman, Anne-Katrin Emde, Vaidehi Jobanputra, Mayu Frank, and Robert B. Darnell

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2. Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays

Jonathan Serrano and Matija Snuderl

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3. Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens

Amit D. Gujar, Diane D. Mao, John B. Finlay, and Albert H. Kim

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Joshua Frenster and Dimitris Placantonakis

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5. Isolation of GBM Stem Cells with Flow Cytometry

Julio Inocencio, Joshua Frenster, and Dimitris Placantonakis