HDAC/HAT Function Assessment and Inhibitor Development Methods and Protocols [Paperback]

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls.

Part I: Analysis of Cellular Processes that Are Regulated by HDACs

 

1. Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells

Anja G?der, Nisintha Mahendrarajah, and Oliver H. Kr?mer

2. Assessment of HDACi-Induced Protein Cleavage by Caspases

Fabian Treude, Tobias Gladbach, Jacqueline Plaster, and J?rg Hartkamp

3. Assessment of HDACi-Induced Cytotoxicity

Lisa Bl?mel, Christian Marx, Marie K?hne, and J?rgen Sonnemann

4. Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization

Britta Unruhe-Knauf and Shirley K. Knauer

5. Analysis of Histone Deacetylase-Dependent Effects on Celll#&