Heterologous Expression of Membrane Proteins: Methods and Protocols [Paperback]

This detailed volume encompasses chapters from leadingexperts in the area of membrane proteins who describe step-by-step protocols developed these last few years to improve the functionalproduction and stabilization of recombinant integral membrane proteins (IMPs). Membraneproteins play a key role in numerous pathologies such as cancer, cysticfibrosis, epilepsy, hyperinsulinism, and Alzheimers disease, yet studies onthese and other disorders are hampered by a lack of information about theproteins involved. This book sets out to aid researchers in rectifying thissituation. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.

Authoritative and up-to-date, HeterologousExpression of Membrane Proteins: Methods and Protocols, Second Editionserves as an ideal guide for scientists attempting to delve deeper into the myriadunique IMP structures.

1. Cell-Free Production of MembraneProteins in Escherichia coli Lysatesfor Functional and Structural Studies

            Ralf-Bernhardt Rues, Erik Henrich,Coilin Boland, Martin Caffrey, and Frank Bernhard

 

2. Membrane Protein Production in theYeast, S. cerevisiae

            Stephanie P. Cartwright, LinaMikaliunaite, and Roslyn M. Bill

 

3. Membrane Proteins Production in Escherichia coli: Protoló´