Sphingolipids as Signaling and Regulatory Molecules [Hardcover]

This book attempts to analyze the latest discoveries in sphingolipid biology and how the alteration of their metabolism leads to altered signaling events and to the development of pathobiological disorders, such as cancer, cardiovascular diseases, asthma, diabetes, inflammation and infectious diseases.

This book examines the latest discoveries in sphingolipid biology and how the alteration of their metabolism leads to altered signaling events and to the development of pathobiological disorders, such as cancer, cardiovascular diseases, asthma and diabetes.

1. An Overview of Sphingolipid Metabolism: From Synthesis to BreakdownChristopher R. Gault, Lina M. Obeid and Yusuf A. HannunAbstractSphingolipid Properties in MembranesDe Novo Synthesis in the ERCeramide Transport from the ER to the GolgiSynthesis of Complex SphingolipidsCeramide Kinase and Ceramide?1?PhosphateCatabolizing Complex Sphingolipids and Sphingomyelins into CeramideThe Catabolism of Ceramides and the Final Common Breakdown PathwayConclusion2. Sphingolipid TransportLaura Riboni, Paola Giussani and Paola VianiAbstractIntroductionIntramembrane Sphingolipid MovementsIntermembrane Sphingolipid TransportConclusion3. Sphingolipid Analysis by High Performance Liquid Chromatography?Ta ndem Mas Spectrometry (HPLC?MS/MS)Jacek Bielawski, Jason S. Pierce, Justin Snider, Barbara Rembiesa, Zdzislaw M. Szulc and Alicja BielawskaAbstractIntroductionSphingolipids: Structure and CompositionLC?MS Methods for Detection and Analysis of Bioactive SphingolipidsLipidomic ApproachSample PreparationAnalysis of Intact Sphingolipids by Mass SpectrometryMechanism of Electrospray Ionization Mass Spectrometry (ESI/MS)MS Scan ModesSpecific Scan Modes for MS/MS InstrumentationSphingolipid IdentificationHPLC?MS/MS MethodologyQuantitationSelection of Internal Standards (ISs)Quantitative CalibrationData HandlingAlternative MethodologyConclusion4. Ceramide Synthases: Roles in Cell Physiology and SignalingJohnny Stiban, RotelC¶