High Throughput Screening: Methods and Protocols [Hardcover]

This third edition volumeexpands on the previous editions both by presenting more detailed protocols forthe techniques described in the first and second editions of High ThroughputScreening: Methods and Protocolsand by covering important new procedures. The first chapter ofthis book provides an overview of important assay development techniques, whilethe rest of the chapters detail how to develop and execute screens at whateverthroughput the user needs. Some chapter examples are: structure-based virtualscreening,  high throughput screening using mass spectrometry, identification of state-dependentblockers for voltage gated calcium channels, bioluminescence resonanceenergy transfer platform to monitor protein-protein interactions in live cells,high throughput flow cytometry, and application of imaging-based assays in microplate formats for highcontent screening. Written in the highly successful Methods in MolecularBiology series format, chapters include introductions to their respectivetopics, lists of the necessary materials and reagents, step-by-step, readilyreproducible laboratory protocols, and tips on troubleshooting and avoidingknown pitfalls.

Cutting edge and thorough, High Throughput Screening: Methods and Protocols,Third Edition, is a valuable resource for anyone who is interested in HTSresearch.

1. Design and Implementation of High Throughput Screening Assays
David J. Powell, Robert P. Hertsberg,and Ricardo Macarr?n

2. Characterization of InhibitorBinding Through Multiple Inhibitor Analysis: A Novel Local Fitting Method
Thomas V. Riera, Tim J. Wigle, andRobert A. Copeland

3. High Throughput Screening UsingMass Spectrometry within Drug Discovery
Mattias Rohman andl“W