Intermediate Filament Proteins [Hardcover]

Intermediate Filament Proteins, the latest volume in the Methods in Enzymology series covers all the intermediate filaments in vertebrates and invertebrates, providing a unique understanding of the multiple different tissue-specific intermediate filaments.

This volume also covers the latest methods that are currently being used to study intermediate filament protein function and dynamics. It will be an important companion for any experimentalist interesting in studying this protein family in their cell or organism model system.

• Focuses on intermediate filaments, including the latest information
• Provides an up-to-date understanding of the field
• Contains contributions from the major scientists working and publishing in the field

Part I:? General Methods to Study IF Proteins
1.?How to Study Intermediate Filaments in Atomic Detail
Anastasia A. Chernyatina, John F. Hess, Dmytro Guzenko, John C. Voss and Sergei V. Strelkov
2.?Mechanical Properties of Intermediate Filament Proteins
Elisabeth E. Charrier and Paul A. Janmey
3.?Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues
Nicole Schwarz, Marcin Moch, Reinhard Windoffer and Rudolf E. Leube
4.?Phospho-Specific Antibody Probes of Intermediate Filament (IF) Proteins
Hidemasa Goto, Hiroki Tanaka, Kousuke Kasahara? and Masaki Inagaki
5.?Assays for Post-Translational Modifications of Intermediate Filament Proteins
Natasha T. Snider and M. Bishr Omary
6.?Immunofluorescence and Immunohistochemical Detection of Keratins
Cornelia Stumptner, Margit Gogg-Kamerer, Christian Viertler, Helmut Denk and Kurt Zatloukal
7.?High Throughput Screening for Drugs that Modulate Intermediate Filament Proteins