Protein Expression in Mammalian Cells: Methods and Protocols [Hardcover]

Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease.? In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

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Protein Expression in Mammalian Cells presents a compendium of vital techniques to further our knowledge of mammalian protein expression.?Each chapter includes a brief introduction, step-by-step, readily reproducible laboratory protocols, and troubleshooting tips.

1. Why Proteins in Mammalian Cells?

??????????? James L. Hartley

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2. Large Scale Transfection of Mammalian Cells

??????????? Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm

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3. Selection of High Expressing Mammalian Cells by Surface Display of Reporters

??????????? Christine T. DeMaria

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4. Expression of a Secreted Protein in Mammalian Cells Usinló&