Antifungal Agents [Paperback]

A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists and molecular biologists the productive tools they need today to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.A collection of state-of-the-art molecular methods for studying antifungal resistance, for discovering and evaluating both new and existing antifungal drugs, and for understanding the host response and immunotherapy of such agents. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Antifungal Agents: Methods and Protocols offers clinician-scientists, microbiologists and molecular biologists the productive tools they need today to understand and successfully develop new therapeutic agents for yeast, mold, and fungal infections.Part I. IntroductionSusceptibility Testing Methods of Antifungal AgentsErika J. ErnstPart II. Antifungal ResistanceDeoxyribonucleic Acid Fingerprinting Methods for Candida SpeciesShawn R. Lockhart, Claude Pujol, Andrew R. Dodgson, and David R. SollCARE-2 Fingerprinting of Candida albicans IsolatesJoachim Morschh?user, Peter Staib, and Gerwald K?hlerTargeted Gene Deletion in Candida albicans Wild-Type Strains by MPAR FlippingJoachim Molƒs