Calcium Signaling Protocols [Hardcover]

The regulation of intracellular Ca²⁺ has continued to be a powerful area of study since the publication of the first and second editions of Calcium Signaling Protocols, and the developments in the field have also, naturally, continued.? With the third edition, expert contributors explore some of the exciting new molecular techniques that have both enabled new studies of intracellular Ca²⁺ regulation and provided much new information on processes.? Comprised of five main section, the book covers theoretical and very simple suspension-based fluorimetric assays, specialist measurement systems, measurement of channel activity, measurement of store release, as well as specialist measurement techniques which include targeted probes, using G-protein chimeras to force Ca²⁺ signalling for screening, and genetically encoded sensors.? Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Updated and accessible, Calcium Signaling Protocols, Third Edition will be a valuable reference for all those contemplating a move into the study of intracellular Ca²⁺.This book explores new protocols and features revised and improved methodologies, making it a valuable reference for all those contemplating a move into the study of intracellular Ca2+. It features lab-ready procedures and includes key implementation advice.

Part I: General

1. Fluorescent Measurement of [Ca²⁺]_c: Basic Practical Considerations

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2. Measurement of [Ca