Cellular and Subcellular Nanotechnology: Methods and Protocols [Hardcover]

In Cellular and Subcellular Nanotechnology: Methods and Protocols expert researchers in the field detail the most recent advances which have been made in utilizing the enormous potential of nanotechnology for probing, imaging and manipulating life on a cellular and subcellular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

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Authoritative and Practical, Cellular and Subcellular Nanotechnology: Methods and Protocols seeks to aid scientists in the further study of applying nanotechnology to all areas of biomedical sciences.

1. Nanoparticle-GFP Chemical Nose Sensor for Cancer Cells Identification

Daniel F. Moyano and Vincent M. Rotello

2. A Method to Map Spatiotemporal pH Changes in a Multicellular Living Organism using a DNA Nanosensor

Sunaina Surana and Yamuna Krishnan

3. A Simple Method to Visualize and Assess the Integrity of Lysosomal Membrane in Mammalian Cells using a Fluorescent Dye

Syed K. Sohaebuddin and Liping Tang

4. Gold Nanoparticle as a Marker for Precise Localization of Nano-objects within Intracellular Sub-Domains

Valeriy Lukyanenko and Vadim Salnikov

5. Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation

Ari Nowacek, Irena Kadiu, Jolc®