Contrast Techniques in Light Microscopy [Paperback]

Contrast in an image is essential to distinguish features from one another and from the background. This practical handbook describes the ways in which light interacts with the specimen in the microscope and the manner in which this is used to generate coIntroduction; Fundamental considerations; Bright-field and dark-ground techniques; Refraction differences between specimen and mountant; the 'Becke Line' ; Polarized-light techniques; Phase contrast and modulation contrast; Interference contrast; Fluoresc