The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.Edward A. Dennis: University of California, San Diego Distinguished Professor of Chemistry and Biochemistry and former Department Chair and Professor of Pharmacology in the School of Medicine, Dennis is currently Editor-in-Chief of the Journal of Lipid Research and Director of the LIPID MAPS initiative. He also served as Chair of the Board of Directors of the Keystone Symposia on Molecular and Cellular Biology and current as an Emeritus member of the Board of Directors. His current research focus is on phospholipases, cell signaling and lipidomics on which he has published over 300 papers, most related to signal transduction by lipid messengersPraise for the Series The Methods in Enzymology series represents the gold-standard. --NEUROSCIENCE Incomparably useful. --ANALYTICAL BIOCHEMISTRY It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page. --BIO/TECHNOLOGY The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection. --CHEMISTRY IN INDUSTRY The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of sel