DNA Microarrays: Gene Expression Applications [Paperback]

This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book.This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book. It also provides detailed protocols as well as many helpful hints to achieve experimental success and to avoid pitfalls.1 DNA Arrays for Expression Measurement: An Historical Perspective.- 1 Introduction.- 2 The Forerunners: Colony Filters and Dot Blots.- 3 High-Density Filters.- 4 Colony Filters and Expression Analysis (Qualitative).- 5 Quantifying Expression with High-Density Filters.- 6 Miniaturisation: cDNA Microarrays.- 7 Miniaturisation: Oligonucleotide Chips.- 8 Data Acquisition, Storage and Mining.- 9 A (Provisional) Conclusion.- References.- 2 Expression Profiling with cDNA Microarrays: A Users Perspective and Guide.- 1 Introduction.- 2 Expression Profiling with cDNA Microarrays: The Basics.- 3 Production of cDNA Microarrays.- 4 Conclusions.- 5 Protocols.- References.- 3 cDNA Microarrayl³¶