Forensic DNA Typing Protocols [Paperback]

A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.A state-of-the-art collection of readily reproducible laboratory methods for DNA identity analysis, including Y chromosome haplotyping, mtDNA, and SNP typing. The book offers well-tested protocols for DNA quantification using real-time PCR on forensic samples and for the determination of the number of amelogenine gene copies. For forensic geneticists, there are readily reproducible methods for species identification, ancient DNA, and pharmacogenetics. Additional chapters address new applications in the forensic genetics lab, such a species identification or typing of CYP polymorphisms for the analysis of adverse to drugs.Forensic DNA-Typing Technologies: A ReviewAngel Carracedo and Paula S?nchez-DizDNA Extraction and Quantitation of Forensic Samples Using the Phenol-Chloroform Method and Real-Time PCRSilvano K?chl, Harald Niederst?tter, and Walther ParsonA Real-Time PCR Protocol to Determine the Number of Amelogenine (X-Y) Gene Copies From Forensic DNA SamplesAntonio Alonso and Pablo Mart?nSpecies Determination: The Role and Use of the Cytochrome b GeneAdrian Linacre and James Chun-I LeeConstructing STR Mulitplex AssaysJohn M. ButlerY Chromosome STR TypingLeonor Gusm?o and C?ntia AlvesUsing Online Databases for Developing SNP Markers of Forensic InterestChristopher PhillipsSNP Typing in Forensic Genetics: A ReviewBeatriz Sobrino and Angel CarracedoGlƒs