Human Cell Culture Protocols [Hardcover]

A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular Medicine series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.Establishment and Maintenance of Normal Human Keratinocyte CulturesClaire LingeCultivation of Normal Human Epidermal Melanocytes in the Absence of Phorbol EstersMei-Yu Hsu, Ling Li, and Meenhard HerlynIsolation and Culture of Human OsteoblastsAlison Gartland, Katherine A. Buckley, Jane P. Dillon, Judith M. Curran, John A. Hunt, and James A. GallagherHuman Osteoclast Culture from Peripheral Blood Monocytes: Phenotypic Characterization and Quantitation of ResorptionKatherine A. Buckley, Benjamin Y. Y. Chan, William D. Fraser, and James A. GallagerHuman Chondrocyte Cultures as Models of Cartilage-Specific Gene RegulationMary B. GoldringHuman Myoblasts and Muscle-Derived SP CellsGrace K. Pavlath and Emanuela GussoniCell Cultures of Autopsy-Derived FibroblastsVolker Meske, Frank Albert, and Thomas G. Ol³&