In Vitro Neurochemical Techniques [Paperback]

Characterization of Receptors by Radiolabeled Ligand Binding Techniques, Martin Davies and Susan M. J. Dunn. Electrophysiological Studies on Receptors In Vitro, Qi-Ying Liu, Anne E. Schaffner, and Jeffrey L. Barker. Subtractive Hydridization: A Method to Identify Novel Genes Induced During Neuronal Programmed Cell Death, H. Michael Tucker and Steven Estus. Messenger RNA Differential Display: An Unbiased Tool to Uncover Alterations in Gene Expression Underlying Biological Processes, V?ronique A.-M. I. Tanay and Alan N. Bateson. Localization of mRNAs in Brain Sections by in situ Hybridization Using Oligonucleotide Probes, Alan N. Bateson. Multiprobe Oligonucleotide Solution Hybridization for the Determination of Relative mRNA Levels, Alan N. Bateson and V?ronique A.-M. I. Tanay. Competitive RT-PCR to Quantitate Steady-State mRNA Levels, Dennis R. Grayson and Snezana Ikonomovic. A Novel PCR-RFLP Detection Method Using an Optimized Set of Restriction Enzymes, A. Petronis, A. Timinskas, V. Basile, A. Vincente, A. Janulaitis, and J. L. Kennedy. Western Blot Analysis, D. D. Mousseau and V. L. Raghavendra Rao. The Phosphoinositide Signal Transduction System, Richard S. Jope, Xiaohua Li, and Mary A. Pacheco. Protein Phosphorylation, Gail V. W. Johnson and Penny K. Davis. Immunoprecipitation of Receptors, Paul L. Chazot, Simon Pollard, and F. Anne Stephenson. Index.In Vitro Neurochemical Techniques is the third work updating and expanding the best-selling inaugural volume of Humana Press's warmly received Neuromethods series, General Neurochemical Techniques (vol. 1). The key techniques detailed in this new edition encompass the breadth of neurochemical and molecular neurobiology research, ranging from the isolation of neuronal genes and the study of their expression to the analysis of receptor-ligand interactions, to the characterization of the consequences of receptor activation. The methods include electrophysiological techniques to explore the functional properties of recló2