Lipid Signaling Protocols [Hardcover]

In this second edition methods are described to measure the synthesis of lipids such as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly characterize the various kinases and phosphatases that regulate the intracellular metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers through detailed experimental protocols, which are complimented by review chapters that highlight the technical considerations, challenges and potential pitfalls associated with using these laboratory-based approaches. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

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1. Method for Assaying the Lipid Kinase Phosphatidylinositol-5-phosphate 4-kinase ? in Quantitative High-Throughput Screening (qHTS) Bioluminescent Format

Mindy I. Davis, Atsuo T. Sasaki, and Anton Simeonov

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2. Assaying Ceramide Synthase Activity In Vitro and in Living Cells using Liquid Chromatography-Mass Spectrometry

Xin Ying Lim, Russell Pickford, and Anthony S Don

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3. Fluorescent Assays for Ceramide Synthase Activity

Timothy A Couttas and Anthony S Don

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4. Identification of the Interactome of a Palmitoylated Membrane Protein, Phosphatidylinositol 4-kinase type II Alpha

Avanti Gokhale, Pearl V. Ryder, Stephanie A. Zlatic, and Victor Faundez

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5. Measurement of LonlC¶