Metabonomics: Methods and Protocols [Hardcover]

This volume on metabonomics provides detailed information on the procedures involved in nuclear magnetic resonance (NMR) spectroscopy, gas chromatography-mass spectrometry (GS-MS), liquid chromatography-mass spectrometry (LC-MS), and capillary electrophoresis-mass spectrometry (CE-MS). Chapters focus on technologies and chemometrics, generation of metabonomics data, extraction of meaningful information from data, drug development, toxicology, diagnostics, and describing metabonomics as an essential part of systems biology. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

1. Metabonomics - Analytical Techniques and Associated Chemometrics at a Glance

Jacob T. Bjerrum

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2. Sample Collection and Preparation of Biofluids and Extracts for NMR Spectroscopy

Gw?na?lle Le Gall

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3. NMR Spectroscopy of Biofluids and Extracts

Gw?na?lle Le Gall

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4. High-resolution Magic-Angle-Spinning NMR Spectroscopy of Intact Tissue

Guro F. Giske?deg?rd, Maria D. Cao, and Tone F. Bathen

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5. Sample Collection and Preparation of Biofluids and Extracts For Liquid Chromatography-Mass Spectrometry

Peiyuan Yin, Lina Zhou, Xinjie Zhao,? and Guowang Xu

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6. Liquid Chromatography-Mass Spectrometry of Biofluids and Extracts

Xinjie Zhao, Lina Zhou, Peiyuan Yin, and Guowang Xu

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7. Sample Collection and Preparation of Biofluids and Extracts For Gas-Chromatography-Mass Spectl³'