Metalloproteins: Methods and Protocols [Hardcover]

Metalloproteins are involved in many key biological processes such as gas transport and metabolism, photosynthesis, cell respiration, the Krebs cycle and many other vital redox reactions. Metalloproteins: Methods and Protocols addresses multiple aspects of metalloenzyme research from the production and purification of metalloproteins using both standard recombinant techniques and a cell-free system, to the electrochemical analysis of metalloproteins, as well as IR techniques, M?ssbauer spectroscopy and the theoretical interpretation of metalloenzyme catalytic and redox processes, to name a few topics. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Metalloproteins: Methods and Protocols seeks to serve both professionals and novices interested in the metalloprotein field.

1. Advances in Research on Metalloproteins

??????????? Perry A. Frey

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2. Purification of O2-Sensitive Metalloproteins

??????????? Carlos Echavarri-Erasun, Simon Arragain, and Luis M. Rubio

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3. Expression and Purification of NifB Protein from Aerobic and Anaerobic Sources

??????????? Carlos Echavarri-Erasun, Simon Arragain, Alessandro A. Scandurra, and Luis M. Rubio

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4. Techniques for the Production, Isolation and Analysis of Iron-Sulfur Proteins

??????????? Jason C. Crack, Jeffrey Green, Andrew J. Thomson, and Nick E. Le Brun

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5. Cell-Free Synthesis of the H-cluster: a Model for the In Vitro Assembly of Metalloprotein Mlă‰