Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols [Hardcover]

The rapid pace of microRNA (miRNA) research continues to drive the advances of techniques for miRNA expression profiling, and innovative technologies that are more sensitive, specific, quantitative, and that are compatible with a wide range of biospecimens have been developed during the past few years.? In Next-Generation MicroRNA Expression Profiling Technology: Methods and Protocols, expert researchers in the field contribute detailed examinations of the most current approaches being used today.? This volume includes comprehensive coverage of methodologies that have been developed for miRNA profiling, as well as next-gen sequencing technology, miRNA databases, and specialized applications, such as cancer studies and miRNA-based non-invasive biomarker development.? Written in the highly successful Methods in Molecular Biology series format, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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The rapid pace of research in this field continues to drive technical advances that make methods more sensitive and applicable. This volume features many new methodologies described by leading researchers, including reproducible laboratory protocols.

1. Controlling miRNA Regulation in Disease

??????????? Willemijn M. Gommans and Eugene Berezikov

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2. Introduction to miRNA Profiling Technologies and Cross-Platform Comparison

??????????? Sarah Aldridgl³’