Patch-Clamp Methods and Protocols [Hardcover]

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively.

The volume includes an extensive theoretical treatise concerning single channel kinetic analysis.

Patch Clamp Methods and Protocols surveys the typical patch clamp applications and advises scientists on identifying problems and selecting the best technique in each instance. The experiments described require a basic level of electrophysiological training, and aid the researcher in pursuing new areas of electrophysiology and using the patch clamp technique effectively. Patch Clamp Methods and Protocols is organized by the major areas of patch clamp application: Pharmacology, Physiology, and Biophysics. Individual techniques are explored within the area that they are applied most often. Researchers will find this text to be an invaluable aid in the design and execution of a wide variety of patch clamp experiments, both on their own and in conjunction with other state-of-the-art methodologies.

Table of contentsPart 1. Pharmacology1. Pharmacological analysis of recombinant NR1a/2A and NR1a/2B NMDAreceptors using whole-cell patch clamp method. Laszlo Fodor and JozsefNagy2. Memantine as an example of a fast, voltage-dependent, open channelN-methyl-D-aspartate (NMDA) receptor blocker. Chris G. Parsons and KateGilling3. Methods for evaluation of positive allosteric modulators ofglutamate AMPA receptors. Edward R. Siuda, Jennifer C. Quirk and EricS. Nisenbaum4. Automated voltage clamp technique. Andrea Ghetti, Antonio Guia andJia Xu5. Flip-the-Tip: automated patch clamping based on glass electrodes.Michael Fejtl, Uwe Czubayko, Alexander H?mmer, Tobias Krauter andAlbrecht Lepple-Wienhues6. The Roboocyte: Automated lƒs