RNA Interference: Challenges and Therapeutic Opportunities [Paperback]

RNA Interference: Challenges and Therapeutic Opportunities provides readers with recent advances in siRNA design, delivery, targeting and methods to minimize siRNAs unwanted effects. Preclinical and clinical use of synthetic siRNAs, the roles of miRNAs in cancer and the promise of extracellular miRNAs for diagnosis are also covered in this meticulous collection, along with novel methods for identifying endogenous siRNAs and the annotation of small RNA transcriptomes. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.

1. RNA Interference: Mechanisms, Technical Challenges, and Therapeutic Opportunities

??????????? Mouldy Sioud

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2. Nanostructured RNAs for RNA Interference

??????????? Yuko Nakashima, Naoko Abe, Yoshihiro Ito, and Hiroshi Abe

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3. One Long Oligonucleotide or Two Short Oligonucleotides Based shRNA Construction and Expression

??????????? Xue-jun Wang and Sheng-qi Wang

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4. PLGA Microspheres Encapsulating siRNA

??????????? Giuseppe De Rosa and Giuseppina Salzano

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5. Magnetic Nanoparticle and Magnetic Field Assisted siRNA Delivery In Vitro

??????????? Olga Mykhaylyk, Yolanda Sanchez-Antequera, Dialechti Vlaskou, Maria Belen Cerda, Mehrdad Bokharaei, Edelburga Hammerschmid, Martina Anton, and Christian Plank

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6. Cytoplasmic Delivery of siRNAs to Monocytes and Dendritic Cells lm