This volume contains information on analyzing sphingolipids, sphingolipid transport and trafficking, and sphingolipid-protein interactions and cellular targets. Its companion Volume 311 presents methods used in studying enzymes of sphingolipid biosynthesis and turnover, including inhibitors of some of these enzymes, genetic approaches, and organic and enzymatic syntheses of sphingolipids and analogs.
The critically acclaimed laboratory standard for more than forty years,
Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Section I: Methods for Analyzing Sphingolipids
[1]: Analysis of Sphingoid Bases and Sphingoid Base 1-Phosphates by High-Performance Liquid Chromatography
[2]: Enzymatic Method for Measurement of Sphingosine 1-Phosphate
[3]: Ceramide Mass Analysis by Normal-Phase High-Performance Liquid Chromatography
[4]: Quantitative Determination of Ceramide Using Diglyceride Kinase
[5]: Analysis of Sphingomyelin, Glucosylceramide, Ceramide, Sphingosine, and Sphingosine 1-Phosphate by Tandem Mass Spectrometry
[6]: Analyses of Glycosphingollpids by High-Performance Liquid Chromatography
[7]: Sphingolipid Extraction and Analysis by Thin-Layer Chromatography
[8]: Extraction and Analysis of Multiple Sphingolipids from a Single Sample
[9]: Purification of Sphingolipid Classes by Solid-Phase Extraction with Aminopropyl and Weak Cation Exchanger Cartridges
[10]: Ganglioside Analysis by Higl³4