Structural Proteomics: High-Throughput Methods [Hardcover]

This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes. Structural Proteomics: High-Throughput Methods, Second Edition begins by exploring the resources available for curation, annotation, and structure prediction in silico, and continues with methods for sample preparation of both proteins and crystals, as well as structural characterization techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and up-to-date, Structural Proteomics: High-Throughput Methods, Second Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.

Part I: Bioinformatics and Data Management

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1. Protein Structure Annotation Resources

??????????? Margaret J. Gabanyi and Helen M. Berman

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2. PiMS: A Data Management System for Structural Proteomics

??????????? Chris Morris

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3. Prediction and Analysis of Intrinsically Disordered Proteins

Marco Punta, Istv?n Simon, and Zsuzsanna Doszt?nyi

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4. I-TASSER Suite: A Standalone Package for Protein Structure Prediction and Structure-Based Protein Function Annotation

??????????? Renxiang Yan, Jianyi Yang, Ambrish Roy, Dong Xu, Jonathan Poisson, and Yang Zhang

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Part II: Soluble Proteins

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