Subcellular Fractionation A Practical Approach [Paperback]

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles.Subcellular Fractionationcontains expert advice and detailed methodology, and will be invaluable to researchers in this important area.

1. Homogenization of tissues and cells
2. Isolation of subcellular fragments
3. Isolation and characterization of nuclei and nuclear subfractions
4. Subcellular fractionation of mitochondria
5. Isolation and characterization of peroxisomes
6. Lysosomes and endocytosis
7. The membranes of the secretory and exocytic pathways
8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells
9. Ribosomes and polysomes
10. Electron microscopy of organelles