T-Cell Development: Methods and Protocols [Hardcover]

This volume provides simple and accessible experiment protocols to explore thymus biology. T-Cell Development: Methods and Protocols is divided into three parts presenting short reviews on T cell development, analysis strategies, protocols for cell preparation, flow cytometry analyses, and multiple aspects of thymocyte biology. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

?Concise and easy-to-use, T-Cell Development: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Part I: Background Information

1. 200 Million Thymocytes and I: A Beginners Survival Guide to T cell Development

Melanie S. Vacchio, Thomas Ciucci and R?my Bosselut

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2. Development of gd T Cells, the Special Forces Soldiers of the Immune System

David L. Wiest

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Part II: Core Approaches and Strategies

3. Genetic Tools to Study T Cell Development

Thomas Ciucci, Melanie S. Vacchio and R?my Bosselut

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4. Assessment of T cell Development by Flow Cytometry

Jan Y.M. Lee and Paul E. Love

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5. Flow Cytometry Analysis of Thymic Cells and their Subpopulations

Izumi Ohigashi and Yousuke Takahama Epithelial

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6. Identifying the Spatial Relationships of Thymic Stromal and Thymocyte Subsets by Immunofluorescence Analysis

Virginia Bain and Ellen R. Richie

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7. Purification of Thymocyte and T Cell Subselóˇ