The PCR Revolution Basic Technologies and Applications [Paperback]

Examines the latest innovations and the overall impact of PCR on areas of molecular research.More than 50,000 researchers in the United States use polymerase chain reaction (PCR) replication technology, and yet a book has not been published on the subject in more than 10 years. This book examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research.More than 50,000 researchers in the United States use polymerase chain reaction (PCR) replication technology, and yet a book has not been published on the subject in more than 10 years. This book examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research.The invention of the polymerase chain reaction (PCR) won the Nobel Prize in Chemistry in 1994 and remains one of the most important scientific discoveries of the twentieth century. More than 50,000 researchers in the United States use PCR replication technology, and yet a book has not been published on the subject in more than ten years. In this book, Dr. Stephen A. Bustin, a world-renowned PCR expert, examines in detail the latest innovations and the overall impact of PCR on many areas of molecular research. The book contains personal reflections, opinions, and comments by leading authorities on the many applications of the PCR and how this technology has revolutionized their respective areas of interest. This book conveys the ways in which PCR has overcome many obstacles in life science and clinical research and also charts the PCRs development from time-consuming, low throughput, non-quantitative procedure to todays rapid, high throughput, quantitative super method.Part I. Basic Technologies: 1. Real time PCR Mickey Williams; 2. Thermostable enzymes used in PCR Sudip K. Rakshit; 3. Inventing molecular beacons Fred Russell Kramer, Salvatore A. E. Marras and Sanjay Tyagi; 4. Rapid PCR and melting analysis Carl T. Wittwer, Randy P. Rasmussen and Kirk M. Ririe; 5. PCR and fluorls8