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In Virus Hybrids as Nanomaterials: Methods and Protocols expert researchers in the field detail many of the methods used to study virus for medial and nonmedical applications.?These include methods and techniques for?genetically engineering viruses for therapeutic purpose and vaccine production, chemically modified viruses for virus-templated nanoparticles production, and genetically engineered or chemically modified viral particles as imaging agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Virus Hybrids as Nanomaterials: Methods and Protocols seek to aid new researchers to get involved in this multidisciplinary area.
Part I: Virus Hybrids as Therapeutic/Vaccine Materials
1. Genetic Engineering and Chemical Conjugation of Potato Virus X
Karin L. Le, Kerstin Uhde-Holzem, Rainer Fischer, Ulrich Commandeur, Nicole F. Steinmetz
2. Genetically Engineering Adenoviral Vectors for Gene Therapy
Lynda Coughlan
3. Simulated Digestion for Testing the Stability of Edible Vaccine, Cucumber mosaic virus (CMV) Chimeric Particle Display Hepatitis C virus (HCV) Peptide
Vitti Antonella, Nuzzaci Maria, Condelli Valentina, Piazzolla Pasquale
4. Using Phage as a Platform to Select Cancer Cell Targeting Peptides
Xin Li, Chuanbin Mao
5. Bacteriophage T4 Capsid Packaging and Unpackaging of DNA and Proteins
Julienne M. Mullaney and Lindsay W. Black
Part II: Virus as building Block
6. TelC6
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