B Cell Receptor Signaling: Methods and Protocols [Hardcover]

This volume looks the current advanced protocols used to study aspects of the B cell receptor (BCR). The chapters in this book cover topics such as the mutant of BCR repertoire to understand antibody evolution; interactions between B cells and viruses; mechanical force during BCR activation; B cell signaling using flow cytometry; confocal microscopy, total internal reflection microscopy and intravital two-photon microscopy; and the methods used to study critical cell components related with B cell activation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, B Cell Receptor Signaling: Methods and Protocols is a valuable resource for everyone in the scientific community currently working in the B cell field.

Preface&
Table of Contents&
Contributing Authors&

1. Activation-Induced Cytidine Deaminase Aided In Vitro Antibody Evolution
Lili An, Chuan Chen, Ruiqi Luo, Yun Zhao, and Haiying Hang?

2. Analyzing Mouse B-Cell Responses Specific to LCMV Infection
Yaxing Hao, Zhirong Li, Yifei Wang, Xiaobing Liu, and Lilin Ye

3. Expression of Exogenous Genes in Murine Primary B Cells and B Cell Lines Using Retroviral Vectors?
Zhiyong Yang and Christopher D. C. Allen?

4. Biophysical Techniques to Study B Cell Activation: Single Molecule Imaging and Force Measurements?
Ivan Rey, David A. Garcia, Brittany A. Wheatley, Wenxia Song, and Arpita Upadhyaya?

5. DNA-Based Probes for Measuring Mechanical Forces lÄ