Bacterial Cell Wall Homeostasis: Methods and Protocols [Paperback]

This volume bringstogether the most widely used and important protocols currently being employedin researching and understanding bacterial cell wall homeostasis. Chapters in BacterialCell Wall Homeostasis cover a variety of subjects, such as: modernmicroscopy techniques and other biophysical methods used to characterize thesubcellular structure of the bacterial cell wall; high-throughput approachesthat can be used to identify all the genes and proteins that participate in thecorrect functioning of an organisms cell wall; protocols for assayingindividual gene products for specific cell wall functions or identify chemicalswith inhibitory activity against the cell wall; and methods for analyzing the non-proteincomponents of the cell wall and the increasing use of computational approachesfor predicting and modeling cell wall related functions and processes. Writtenin the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary material and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Thoroughand cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocolsemphasizes the diversity of the research taking place in bacterial cell wallhomeostasis, and explains how the integration of information from acrossmultiple disciplines is going to be essential if a holistic understanding ofthis important process is to be obtained.

 

PartI Analyzing Physical Properties of the Bacterial Cell Wall

1.   AtomicForce Microscopic Analysis of Bacterial Cell Wall Peptidoglycan Architecture      Robert D. Turner, Jamie K. Hobbs, andSimon J. Foster       &al§