Laboratory Exercises in Microbiology [Spiral bound]

Laboratory Exercises in Microbiology, tenth Edition was designed and written to be directly correlated to Prescotts Microbiology, tenth Edition, by Joanne M. Willey, Linda M. Sherwood, and Christopher J. Woolverton. The class-tested exercises are modular to allow instructors to easily incorporate them into their course. This balanced introduction to each area of microbiology now also has accompanying Connect content for additional homework and assessment opportunities.

PART ONE Microscopic Techniques

1. Bright-Field Light Microscope (Dark Image, Brighter Background) and Microscopic Measurement of Organisms

2. The Hanging Drop Slide and Bacterial Motility

3. Dark-Field Light Microscope: Bright Image, Dark Background

4. Phase-Contrast Light Microscope

5. Fluorescence Microscope

PART TWO Bacterial Morphology and Staining

6. Negative Staining

7. Smear Preparation and Simple Staining

8. Gram Stain

9. Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures

10. Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)

11. Capsule Staining

12. Flagella Staining: West and Difcos SpotTest Methods

PART THREE Basic Laboratory and Culture Techniques

13. Sterilization and Microbiological Culture Media Preparation

14. Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures

15. Spread-Plate Technique

16. The Streak-Plate Technique: Selective and Differential Media

17. Pour-Plate Technique

18. Cultivation of Anaerobic Bacteria

19. Determination of Bacterial Numbers

PART FOUR Biochemical Activities of Bacteria

20. Carbohydrates I: Fermentation and ?-Galactosidase Activity

21. Carbohydrates II: Triple Sugar Iron Agar Test

22. Carbohydrates III: Starch Hydrolysis

23. Lipids: Lipid Hydrolysis

24. Proteins, Amino Acids, and Enzyl³'