Lipases and Phospholipases: Methods and Protocols [Hardcover]

This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.

Part 1. Overview of Lipases, Phospholipases and Esterases.- Lipases: An Overview.- Carbohydrate Esterases: An Overview.- Phospholipases: An Overview.- Part 2. Activity Screening and Expression.- Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates.- A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities using ?-Eleostearic Acid-Containing Substrates.- Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline.- Lipase and Phospholipase Activity Methods for Marine Organisms.- Plant Lipases: Functional Expression of CPLIP1 from Carica papaya.- Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2.- Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural StudielÓ.